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Asia Pacific: Detecting trace amounts of pesticide in less than two hours

Food scientists from the National University of Singapore developed a rapid and highly sensitive screening technique capable of detecting minute amounts of pyrethroids in vegetables, a common type of synthetic pesticide applied on agricultural crops.

The strategy makes use of magnetic nanoparticles to simplify pyrethorid extraction, reducing the screening process to under two hours.

The research team led by Assistant Professor Yang Hongshun from the NUS Food Science and Technology Programme were behind the development of novel magnetic nanoparticles that could speed up the screening of pesticide residue in vegetables

Synthetic pesticides such as pyrethorids are widely used in vegetable farming to control agricultural pests.

While the use of these pesticides improves crop yield, long-term ingestion of excessive pesticide residues through the consumption of fruits and vegetables that have been subject to pesticide abuse could lead to adverse health effects.

Several methods have since been established to screen vegetable crops for pyrethorid residue.

However, they require time consuming and costly procedures such as column filtration and centrifugation, and can take up to six hours to process and analyze a sample.

To simplify the procedures, Assistant Professor Yang and his PhD student, Yu Xi, developed polystyrene coated magnetic nanoparticles that can effectively extract pyrethorid residue from vegetable crops for analysis via simple magnetic separation.

The nanoparticles are first added into a liquid sample obtained from vegetables, serving as ‘micro magnets’ to attract pyrethroid molecules.

Pyrethroids bound on the nanoparticles are then washed off by a small amount of organic solvent and collected for analysis.

This innovation allows analysis to be completed in less than two hours, and is able to detect pyrethroids at a concentration level of as low as 0.02 nanogram per gram of vegetables. The nanoparticles can also be reused up to 30 times.

“With small modifications to the extraction process, this method can be used to detect pyrethroids in other food products such as nuts, fruits and vegetable oils,” said Yu.

“Modifications can also be made to the coating of the nanoparticles to detect other types of pesticides and chemicals including mycotoxins and antibiotics.”

Moving forward, the duo will look into expanding the applications of their novel technique to enable screening of different chemical hazards in various food types.

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