The AOAC Research Institute has certified the DuPont BAX System Real-Time polymerase chain reaction (PCR) Assay for Genus Listeria as a performance tested method (PTM).
This assay has been validated on frankfurters, cooked shrimp, spinach, queso fresco and environmental surfaces using reference method media, as well as proprietary media for a shorter, single-stage enrichment.
The institute, a subsidiary of AOAC International, validated the BAX System method as equivalent to the reference culture method for detecting Listeria species, but with fast time to results.
BAX System assays for detecting E. coli O157:H7, non-O157 STEC, Campylobacter and other pathogens are already PTM-certified.
In addition, AOAC International has awarded Official Method of Analysis (OMA) status to the BAX System method for detecting for Salmonella and Listeria monocytogenes.
“Many customers rely on AOAC certification to ensure their selected pathogen detection method is accurate, reliable and reproducible,” said Morgan Wallace, research microbiologist and validations leader, DuPont Nutrition & Health.
“We are proud to add this new real-time assay for Listeria spp. to the long list of AOAC-approved test methods from DuPont for advanced food diagnostics.”
The system can be used as a quick and reliable method for detecting Listeria in a variety of products.
It combines shorter, simpler sample preparation and faster real-time processing, without sacrificing accuracy or reliability.
The assay can be run in conjunction with real-time PCR assays for L. monocytogenes and Salmonella, allowing users to receive results for all three targets in a little over an hour.