Thermo Fisher Scientific has developed a precise method to determine organic acids in fruit juice and wine samples, which is detailed in an application note available on its website.
Its application note 1068 entitled Determination of Organic Acids in Fruit Juices and Wines by High-Pressure IC demonstrates the separation of 30 anions on a high-resolution anion-exchange column designed to resolve a large number of organic acids and inorganic anions using hydroxide gradient elution.
It says the column is packed with a smaller particle resin than earlier generation columns and pairing it with a high pressure ion chromatography (HPIC) system delivers the best ion chromatography (IC) organic acid resolution to date.
Suppressed conductivity detection offers high sensitivity even at low concentrations.
The specificity of this method allows simple sample treatments without complex extraction or derivatization procedures.
Juice and wine makers monitor organic acid profiles to determine the freshness and quality of their products.
The determination of organic acids also plays an important role when testing the authenticity of fruit juices, especially those that are likely targets for adulteration and dilution procedures.
This method enables separation of a large variety of organic acids with inorganic anions while minimizing interferences.